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P-Aminobenzoic acid

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DL-Citronellol

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Disodium Citriate

Erythorbic Acid

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Neryl Butyrate

Neryl Formate

Ethyl Vanillin

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Ammonium Alginate

(CAS#: 9005-34-9)

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CAS: 9005-34-9

Definition: Ammonium alginate is the ammonium salt of alginic acid.

Chemical Formula: (C 6 H 11 NO 6 ) n

Structural formula:

Use function: Stabilizer, thickener, gelling agent, emulsifier

Solubility: Dissolves slowly in water forming a viscous solution; insoluble in ethanol, and ether

Specification:

Purity
Loss on drying	Not more than 15% (105°, 4h)
Water-insoluble matter	Not more than 2% on the dried basis See description under TESTS.
Sulfated ash	Not more than 7% on the dried basis
Arsenic	Not more than 3 mg/kg (Method II)
Lead	Not more than 5 mg/kg Prepare a sample solution as directed for organic compounds in the Limit Test, using 5 m g of lead ion (Pb) in the control
Microbiological criteria	Total plate count: Not more than 5,000 colonies per gram. Initially prepare a 10 -1 dilution by adding a 50 g sample to 450 ml of Butterfield's phosphate buffered dilution water and homogenizing in a high speed blender. Yeasts and moulds: Not more than 500 colonies per gram Coliforms: Negative by test Salmonella: Negative by test

Test Method:

Precipitate formation with calcium chloride

To a 0.5% solution of the sample in sodium hydroxide TS add one-fifth of its volume of a 2.5% solution of calcium chloride. A voluminous, gelatinous precipitate is formed. This test distinguishes ammonium alginate from gum arabic, sodium carboxymethyl cellulose, carrageenan, gelatin, gum ghatti, karaya gum, carob bean gum, methyl cellulose and tragacanth gum.

Precipitate formation with ammonium sulphate

To a 0.5% solution of the sample in sodium hydroxide TS add one-half of its volume of a saturated solution of ammonium sulfate. No precipitate is formed. This test distinguishes ammonium alginate from agar, sodium carboxymethyl cellulose, carrageenan, de-esterified pectin, gelatin, carob bean gum, methyl cellulose and starch.

Test for alginate

Dissolve as completely as possible 0.01 g of the sample by shaking with 0.15 ml of 0.1 N sodium hydroxide and add 1 ml of acid ferric sulfate TS. Within 5 min, a cherry-red colour develops that finally becomes deep purple.

PURITY TESTS

Water-insoluble matter

Disperse 2 g of the sample, weighed to the nearest 0.1 mg, in 800 ml of water in a 2,000-ml flask. Neutralize to pH 7 with sodium hydroxide TS and then add 3 ml in excess. Add 40 ml of hydrogen peroxide solution containing 30% by weight H 2 O 2 , cover the flask and boil for 1 h with frequent stirring. Filter while hot through a tared Gooch crucible provided with a glass fibre filter (2.4 cm, No. 934 AH, Reeve Angel & Co., Clifton, N.Y., or equivalent filter). If slow filtration is caused by high viscosity of the sample solution, boil until the viscosity is reduced enough to permit filtration. Wash the crucible thoroughly with hot water, dry the crucible and its contents at 105o for 1 h, cool and weigh. Calculate as percentage of the dry weight.

Test method of assay:

Proceed as directed under Carbon Dioxide Determination by Decarboxylation in the General Methods. Each ml of 0.25 N sodium hydroxide consumed is equivalent to 5.5 mg of carbon dioxide (CO 2 ) or 27.12 mg of ammonium alginate (equivalent weight 217).